Sanger & Next-Generation-Sequencing

Next Generation Sequencing (NGS) has revolutionized the study of molecular biology and genomics sciences.

The access to state-of-the-art sequencing technologies of the research community is essential, but is not available for all groups working in the fields.

Therefore, the service group offers its expert assistance services to internal and external collaboration to perform research on functional genomics analyses. Further specific services are developed upon a request particularly within NGS projects and consortia of functional genome analysis of farmed animals.

Übersicht von Sequenzanalysen
Sequenziergerät HiSeq2500
Abbildung einer Sanger-Sequenz

Our Illumina HiSeq2500 and cBot system typically generates single-end reads or paired-end reads up to 280 million clusters (reads) per lane and 8 lanes per a high-output flowcell. We offer sequencing services for DNA and RNA as well as microbial 16S metagenomics. Our services include standard sequencing listed below, but can be also custom or other specific application:

  • RNA-Seq (Total RNA, mRNA, depletion protocols)
  • miRNA-Seq
  • ChIP-Seq
  • DNA methylation sequencing (RRBS und WGBS)
  • 16S/18S-Seq metagenome

Our standard NGS workflow includes sample processing, DNA library preparation and Next Generation Sequencing as well as bioinformatics assistance and advances data analysis.

In addition, we also offer a service for Sanger sequencing to complement the holistic NGS sequencing methods. Templates for standard Sanger sequencing comprise:

  • PCR products
  • Recombinant vector DNA