Reproductive Biology – Experimental Facilities

Sperm from bulls is provided by cooperation partners

2. Investigated materials:

• Oocytes
• Embryos
• Sperm
• Somatic cells
• Tissue and body fluids
  
  

1. Clinical Methods

• Observation of ovarian and uterine features by means of ultrasonography and endoscopy
• Ultrasound guided follicular aspiration in cattle and horses
• Endoscopic oocyte aspiration in gilts and cattle
• Nonsurgical embryo recovery and transfer in cattle
• Endoscopic embryo recovery and transfer in gilts
• Minimal-invasive surgery in cattle and pig
• Endoscopic collection of substrates and tissues relevant for reproduction
• Minimal-invasive application of biological active substances into follicles in pigs and horses
• Catheterisation of periphereal and central blood vessels in cattle and pig

2. Embryological Methods

• In vitro maturation, fertilization and culture
• Micromanipulation
• Intracytoplasmic sperm injection (ICSI)
• Biopsy of blastomeres
• Computer assisted sperm analysis (CASA)

3. Cell physiological Methods

• Cell and tissue culture (2D, 3D)
• Immunohistochemistry
• In situ hybridisation
• Microinjection in somatic and germ cells
• Flowcytometry
• 3-D image cytometry, Densitometry
• Computer aided Image analysis
• Morphological and cytological analysis
• Real-time impedance measurement

4. Biochemical and molecular Methods

• TLC
• HPLC
• Micro-FPLC
• Elektrophoresis (SDS-PAGE, IEF, preparative Elektrophoresis, HPEC, native gel electrophoresis)
• Western blotting
• mRNA-Analysis
• RIA, ELISA
• Beta- and Gammacounting
• EMSA (electrophoretic mobility shift assay)
• IP (Immunoprecipitation)
• ChIP (Chromatin Immunoprecipitation)
• DNA methylation analysis
• RT-PCR
• Chart-PCR (Chromatin accessibility assay by real-time PCR)
• Genome editing (CRISPR/Cas9)