GreenFeed system for methane measurements of cows in free stall barns
Balance tests
Facilities for conducting nutrient balance tests on cattle, pigs and chickens
Chromatography techniques
GC-FID (e.g. volatile fatty acids, alcohols)
GC-MS (e.g. long-chain fatty acids, glucose)
HPLC (e.g. amino acids, peptides, sugar alcohols)
Thin layer chromatography (e.g. preparative isolation of lipids)
Stable isotope techniques
Working with stable isotope labelled nutrients or metabolites to quantify nutrient and energy metabolism in vivo
Using natural isotope abundance (13C, 15N, 18O, 2H) range to track nutrient metabolism
Elemental analysis – isotope ratio mass spectrometry (IRMS) to quantify 13C, 15N abundance in feedstuffs or tissues
Gas chromatography combustion-IRMS to measure isotopic enrichment of individual substances (e.g. 13C abundance in fatty or amino acids)
Gas bench-Continous Flow-IRMS to measure 13C or 18O abundance in breath gas-CO2 or 2H and 18O abundance in biological liquids (e.g. plasma, saliva)
Molecular mass spectrometry (GC-quadrupole-MS in EI, PCI or NCI mode) to track nutrients or metabolites in plasma, urine or tissue (e.g. glucose, keto acids)
Clinical chemistry
Measurement of enzyme and substrate concentrations in blood and urine by means of automated spectrophotometry (e.g. NEFA, glucose)
Cell culture
Primary culture of neurons
Neuronal cell lines
Protein analytics
2D gel electrophoresis
Receptor binding studies
Western blot
Immunoprecipitation
ELISA
Absolute and relative quantification of gene expression