Genome Biology – experimental basis

Animal species:

  • beef (heifers, cows)
  • pig (different breeds)
  • mouse (mice from breeding lines of the FBN)
  • transgenic and knockout mouse lines (IGFBP-2, GHR)
  • poultry (various breeds of chicken) and in ovo experiments
  • fish (Coregonus, zander, trout)

Range of methods

Cell culture laboratory

  • primary cell cultures (pig, chicken, cattle, zander, mouse), cell lines
  • light and fluorescence microscopy
  • confocal laser microscopy
  • live cell imaging
  • computer-aided image processing / image analysis systems
  • three-dimensional image cytometry, densitometry
  • microinjection into somatic cells
  • electrophysiological stimulation of muscle cells

Cell Physiological Methods

  • histology, cryostat microtomy
  • tissue Structure Analysis
  • In situ hybridization (chromosomes, cells and tissues)
  • immunohistochemistry
  • proliferation Assay
  • real time respirometry and glycolytic activity measurements
  • blood analysis

Biochemical / molecular biological methods

  • CRISPR/Cas9 editing of cell lines
  • DNA and RNA isolation, PCR, RT-qPCR, cloning
  • electrophoresis (SDS-Page, IEF, preparative electrophoresis)
  • EMSA (Electrophoretic Mobility Shift Assay)
  • ChIP (chromatin immunoprecipitation)
  • DNA methylation analysis (Pyrosequencing)
  • Sanger sequencing
  • Chart-PCR (chromatin accessibility assay by real-time PCR)
  • reporter assays
  • gene editing
  • western blotting
  • capillary immune electrophoresis
  • metabolite analysis (clinical chemistry, RIA, ELISA, ECLIA)

High throughput analytics

  • Next Generation Sequencing (WGS, RNA-Seq, small RNA-Seq, RRBS, WGBS, 16S-Seq)
  • directed high-throughput gene expression analysis
  • high throughput genotyping
  • project specific genotyping (KASP, Taqman)
  • mass spectrometry
  • multiplex immunoassays