Genome Biology – experimental basis

Animal species:

beef (heifers, cows)
pig (different breeds)
mouse (mice from breeding lines of the FBN)
transgenic and knockout mouse lines (IGFBP-2, GHR)
poultry (various breeds of chicken) and in ovo experiments
fish (Coregonus, zander, trout)

Range of methods

Cell culture laboratory

primary cell cultures (pig, chicken, cattle, zander, mouse), cell lines
light and fluorescence microscopy
confocal laser microscopy
live cell imaging
computer-aided image processing / image analysis systems
three-dimensional image cytometry, densitometry
microinjection into somatic cells
electrophysiological stimulation of muscle cells

Cell Physiological Methods

histology, cryostat microtomy
tissue Structure Analysis
In situ hybridization (chromosomes, cells and tissues)
immunohistochemistry
proliferation Assay
real time respirometry and glycolytic activity measurements
blood analysis

Biochemical / molecular biological methods

CRISPR/Cas9 editing of cell lines
DNA and RNA isolation, PCR, RT-qPCR, cloning
electrophoresis (SDS-Page, IEF, preparative electrophoresis)
EMSA (Electrophoretic Mobility Shift Assay)
ChIP (chromatin immunoprecipitation)
DNA methylation analysis (Pyrosequencing)
Sanger sequencing
Chart-PCR (chromatin accessibility assay by real-time PCR)
reporter assays
gene editing
western blotting
capillary immune electrophoresis
metabolite analysis (clinical chemistry, RIA, ELISA, ECLIA)

High throughput analytics

Next Generation Sequencing (WGS, RNA-Seq, small RNA-Seq, RRBS, WGBS, 16S-Seq)
directed high-throughput gene expression analysis
high throughput genotyping
project specific genotyping (KASP, Taqman)
mass spectrometry
multiplex immunoassays