Metabolomics comprises the characterization of metabolites in organisms, their quantification at different time points and the differentiation of metabolite concentrations under the influence of external stress factors or other modified influencing variables. Progress in the field of genomics, proteomics and transcriptomics is increasingly giving rise to questions that can only be answered by these investigations of primary and secondary metabolism.
Using state-of-the-art mass spectrometry (MS) equipment, the FBN's Metabolomics platform offers the simultaneous analysis, characterization and quantification of hundreds of different metabolites, thus providing the ideal basis for understanding the biological processes of farm animals. Common metabolites are e. g. proteinogenic amino acids, metabolites from the range of organic acids, nonproteinogenic amino acids, carbohydrates and fatty acids. MS databases (GC-MS: NIST database, LC-MS: m/z cloud) are used for the analysis of the spectra, which are constantly being expanded and allow a fast identification of the investigated metabolites.
The choice of the optimal analysis method is essentially determined by the type (hydrophilic properties, molecular weight) of the metabolites to be investigated:
The following criteria must be met:
Possible applications for metabolism studies:
Note: The Metabolomics Plattform is under construction.