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Reproductive Biology: Experimental Facilities
Experimental subjects:
- Species:
- Cattle (heifers, cows)
- Pig (gilts, sows, barrows)
- Mouse (female mice from FBN lines)
- Investigated materials:
- Oocytes
- Embryos
- Sperm
- somatic cells
- Explantates
- tissue and body fluids
Sperm (Cattle & Horse) were obtained from partners in cooperation
Methods:
- Clinical Methods
- Observation of ovarian and uterine features by means of ultrasonography and endoscopy
- Ultrasound guided follicular aspiration in cattle and horses
- Endoscopic oocyte aspiration in gilts and cattle
- Nonsurgical embryo recovery and transfer in cattle
- Endoscopic embryo recovery and transfer in gilts
- Minimal-invasive surgery in cattle, pigs and horses
- Endoscopic collection of reproductive relevant substrates and tissues
- Minimal-invasive application of biological active substances into follicles in pigs and horses
- Catheterisation of periphereal and central blood vessels in cattle, pigs and horses
- Catheterisation of oviducts in pigs
- Microdialysis of ovaries
- Embryological Methods
- Maturation, fertilization and cultivation in vitro
- Intracytoplasmic sperm injection (ICSI)
- Biopsy of blastomeres
- Analysis of sperm
- Cell physiological Methods
- Cell and tissue culture
- Immunohistochemistry
- In situ Hybridisation
- Microinjection in somatic cells
- Flowcytometry
- 3-D image cytometry, Densitometry
- Computer aided Image analysis
- Conductrometry
- Aggrigometry
- Morphological and cytological analysis
- Biochemical and molecular Methods
- TLC
- HPLC
- Micro-FPLC
- Electrophoresis (SDS-Page, IEF, preparative Electrophoresis, HPEC)
- Western blotting
- mRNA-Analysis
- RIA, ELISA, ECLIA
- Radioreceptorassay
- Beta- und Gammacounting
- EMSA (electrophoretic mobility shift assay)
- ChIP (Chromatin Immunoprecipitation)
- DNA methylation analysis
- Chart-PCR (Chromatin accessibility assay by real-time PCR)